Generation of new transgenic tools in zebrafish
AIM & BACKGROUND
Zebrafish is an excellent model for fast and efficient generation of tissue specific transgenic reporters. In our lab we have generated a dataset of candidate gene regulatory elements that drive vascular specific gene expression. In this project you will have the opportunity to generate and test constructs for transgenesis. This will validate if the predicted sequences indeed drive the fluorescent expression in correct tissue.
This project will involve molecular biology techniques, zebrafish handling, microscopy, micromanipulations. The project can be adapted to fit as either an short independent project or a master thesis, according to your preference.